End Test Now
Which one of the following DOES NOT inhibit protein biosynthesis?
Puromycin
Chloramphenicol
Cycloheximide
Oligomycin
The activation of the complement components occurs via three distinct pathways. Which of the following component(s) is specific to the ‘Alternate Pathway’?
Factor B and D
Mannose binding protein
C1qr2s2
C2
Which one of the following enzymes fixes CO2 into organic form?
Ribulose 5-phosphate kinase
Ribulose 1,5-bisphosphate carboxylase
Pyruvate dehydrogenase
Carbonic anhydrase
Cytochrome C is normally found in the inner mitochondrial membrane. It is released into the cytoplasm during
Apoptosis
Necrosis
Cell differentiation
Cell proliferation
Horseradish peroxidase and alkaline phosphatase are the two enzymes commonly utilized as reagents in ELISA, because these enzymes
are colored proteins
are very small
have high turnover number
bind to ELISA plates
The polarity of water molecule is due to
its tetrahedral structure
bonding electrons being attracted more to oxygen
bonding electrons being attracted more to hydrogen
its weak electrolytic property
Cyanide poisoning is due to its direct inhibition of
Electron transport chain
Fatty acid biosynthesis
Fatty acid oxidation
Nucleic acid biosynthesis
In humans, the largest energy reserve is
liver glycogen
muscle glycogen
blood glucose
adipose tissue triacylglycerol
A mixture of four proteins of pIs 11, 7, 5 and 3 are loaded on DEAE anion-exchange column equilibrated with low ionic strength buffer of pH 8. Which of the four proteins would be expected to be retained on the column?
Protein with p1 but not the others
Proteins with pIs 11 and 7 but not 5 and 3
Proteins with pIs 7, 5 and 3
Protein with pI7 but not the others
Valinomycin, a cyclic peptide antibiotic, facilitates the transport of which one of the following ions?
K+
H+
Match P, Q, R and S with the appropriate numbers 1 to 6 on the right
P-5, Q-1, R-6, S-2
P-1, Q-2, R-3, S-4
P-3, Q-4, R-5, S-1
P-2, Q-6, R-1 , S-3
Two purified DNA samples A and B contain equal number of base pairs. Each of these DNA samples has one site each for EcoRI and BamHI restriction enzymes. Complete digestion with both the enzymes yielded 3 DNA bands and 2 DNA bands respectively for A and B upon electrophoresis of the digestion products. Which one of the following explains the observation?
A is circular DNA and B is linear
B is circular DNA and A is linear
A is circular DNA and B could be linear or circular
B is circular DNA and A could be linear or circular
In the following enzyme catalyzed reaction which follows Michaelis-Menten kinetics
Match the items in Group I with those in Group II
P-3, Q-4, R-l, S-2
Q-4, R-l, S-5
P-3, Q-5, R-4, S-l
P-6, Q-5, R-l, S-4
Three samples of antibodies were electrophoresed under denaturing and reducing conditions on a 15 % acrylamide gel, followed by staining with Coomassie blue dye. Samples 1, 2 and 3 showed two, three and four stainable bands respectively. Which one of the following conclusions can be made from these observations?
Sample 1 is IgG, 2 is IgA and 3 is IgM
Sample 1 is IgA, 2 is IgM and 3 is IgG
Sample 1 is IgG, 2 is IgM and 3 is IgA
Sample 1 is IgA, 2 is IgG and 3 is IgM
Four identical PCR reactions were carried out in tubes named I, II, III and IV. Besides the usual mix of dNTPs, each of the tubes respectively contained P dATP, P dATP, p dATP and P rNTP. Which one of the tubes will have radio labeled PCR product?
Tube I
Tube II
Tube III
Tube IV
Match the following
P-5, Q-4, R-2, S-8
P-6, Q-3, R-1, S-7
P-4, Q-2, R-1, S-6
P-1, Q-7, R-5 , S-3
Collagen, a-keratin and tropomyosin have common structural features. They are P) disulfide bridges to neighboring proteins. Q) repeating sequences of amino acids R) a high -sheet content S) superhelical coiling
P,Q
Q,R
Q, S
P,R
P-1, Q-6, R-5, S-4
P-5, Q-7, R-4, S-8
P-2, Q-5, R-3 , S-4
P-3, Q-1, R-2, S-6
Scatchard analysis of ligand-receptor interaction yielded the graph shown below. The affinity of the ligand-receptor interaction can be obtained from
Y intercept
X intercept
Slope of the line
Product of X intercept and Y intercept
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